FBXL5 antibody (Middle Region)

Catalog No. ABIN2774689

The Rabbit Polyclonal anti-FBXL5 antibody (ABIN2774689) specifically detects FBXL5 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Horse and Pig samples.

Quick Overview for FBXL5 antibody (Middle Region) (ABIN2774689)

Target: FBXL5 (F-Box and Leucine-Rich Repeat Protein 5 (FBXL5))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FBXL5 Antibody

Binding Specificity: Middle Region

Sequence: VHWARGDWYS GPATELDTEP DDEWVKNRKD ESRAFHEWDE DADIDESEES

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXL5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXL5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 691 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXL5

Target: FBXL5 (F-Box and Leucine-Rich Repeat Protein 5 (FBXL5))

Alternative Name: FBXL5

Background: FBXL5 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXL5 belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternative splicing of this gene generates 2 transcript variants.
Alias Symbols: FBL4, FBL5, FLR1
Protein Interaction Partner: NABP2, CUL1, SNAI1, SKP1, FBXL5, HERC2, UBC, IREB2, ACO1, RBX1, MUS81, MMS19, FAM96B, ORC4, EP300, SMURF1, BTG1, COPS5, PLK1, CSNK2B, DCTN1,
Protein Size: 691

Molecular Weight: 76 kDa

Gene ID: 26234

NCBI Accession: NM_012161, NP_036293

UniProt: Q9UKA1

Pathways: Transition Metal Ion Homeostasis