FBXO25 antibody (N-Term)

Catalog No. ABIN2774695

This anti-FBXO25 antibody is a Rabbit Polyclonal antibody detecting FBXO25 in WB. Suitable for Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Cow, Rabbit and Pig.

Quick Overview for FBXO25 antibody (N-Term) (ABIN2774695)

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXO25 Antibody

Binding Specificity: N-Term

Sequence: LGEAFNRLDF SSAIQDIRRF NYVVKLLQLI AKSQLTSLSG VAQKNYFNIL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXO25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FBXO25

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXO25

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Alternative Name: FBXO25

Background: FBXO25 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: FBX25, MGC20256, MGC51975
Protein Interaction Partner: SYTL2, ING3, TMA7, HP1BP3, ADRBK1, PRRG1, PRKG2, PRKCA, PPP1R8, PPID, PDGFRA, PCK1, ODF2, KRT8, KDR, IRS1, DNAJB2, HIP1, GTF2B, FKBP3, ELK1, EGFR, DCX, EID3, PRR15, APLF, TCEANC, WDSUB1, TRUB1, TCEAL2, TCP10L, ANKRD13A, C7orf50, RHBDD1, NSRP1, RABEP2, C11
Protein Size: 291

Molecular Weight: 34 kDa

Gene ID: 26260

NCBI Accession: NM_012173, NP_036305

UniProt: Q8TCJ0