RAD18 antibody (Middle Region)

Catalog No. ABIN2774765

This anti-RAD18 antibody is a Rabbit Polyclonal antibody detecting RAD18 in WB. Suitable for Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse and Guinea Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for RAD18 antibody (Middle Region) (ABIN2774765)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD18 Antibody

Binding Specificity: Middle Region

Sequence: KQVTKVDCPV CGVNIPESHI NKHLDSCLSR EEKKESLRSS VHKRKPLPKT

Predicted Reactivity: Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RAD18. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAD18

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 495 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RAD18 antibody (ABIN2774765)

Tsuji, Watanabe, Araki, Shinohara, Yamagata, Tsurimoto, Hanaoka, Yamamura, Yamaizumi, Tateishi: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, (2008) (PubMed).

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18

Background: RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RNF73
Protein Interaction Partner: TAX1BP1, UBC, SHPRH, RAD18, SIVA1, USP2, UBE2B, UBE2A, HLTF, PCNA, BPLF1, USP5, DBF4, BRCA1, DZIP3, POLH, DTL, Ankrd32, MLKL, ECT2, RPL10A, TRIM23, PCGF6, TRIM8, RFC2, CPD, SPRTN, ZMYM2, MAPK8, POLN, WRNIP1, gag-pol, ORC2, SUMO2, POLK, NBN, Smc6, HIST1H2A
Protein Size: 495

Molecular Weight: 56 kDa

Gene ID: 56852

NCBI Accession: NM_020165, NP_064550

UniProt: Q9NS91