RNF128 antibody (C-Term)

Catalog No. ABIN2774780

This Rabbit Polyclonal antibody specifically detects RNF128 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse and Rabbit.

Quick Overview for RNF128 antibody (C-Term) (ABIN2774780)

Target: RNF128 (Ring Finger Protein 128 (RNF128))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF128 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF128 Antibody

Binding Specificity: C-Term

Sequence: LEEHVQSTNE SLQLVNHEAN SVAVDVIPHV DNPTFEEDET PNQETAVREI

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF128. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF128

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 428 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF128

Target: RNF128 (Ring Finger Protein 128 (RNF128))

Alternative Name: RNF128

Background: RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: FLJ23516, GRAIL
Protein Interaction Partner: CEP76, APPBP2, TP53, UBE2H, UBE2E1, UBE2D1, Arhgdia, Arhgdib, RNF128, UBE2T, UBE2I, UBE2N, CD40LG, OTUB1, USP8,
Protein Size: 428

Molecular Weight: 47 kDa

Gene ID: 79589

NCBI Accession: NM_194463, NP_919445

UniProt: Q8TEB7