Annexin IV antibody (N-Term)

Catalog No. ABIN2774909

The Rabbit Polyclonal anti-Annexin IV antibody has been validated for WB and IHC. It is suitable to detect Annexin IV in samples from Human, Rat, Mouse, Dog, Pig, Cow, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio). There are 2+ publications available.

Quick Overview for Annexin IV antibody (N-Term) (ABIN2774909)

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Reactivity: Human, Rat, Mouse, Dog, Pig, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Annexin IV Antibody

Binding Specificity: N-Term

Sequence: GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ANXA4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 321 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Annexin IV antibody (ABIN2774909)

de la Cuesta, Alvarez-Llamas, Maroto, Donado, Zubiri, Posada, Padial, Pinto, Barderas, Vivanco: "A proteomic focus on the alterations occurring at the human atherosclerotic coronary intima." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 4, pp. M110.003517, (2011) (PubMed).

Staquicini, Cardó-Vila, Kolonin, Trepel, Edwards, Nunes, Sergeeva, Efstathiou, Sun, Almeida, Tu, Botz, Wallace, OConnell, Krajewski, Gershenwald, Molldrem, Flamm, Koivunen, Pentz, Dias-Neto, Setubal et al.: "Vascular ligand-receptor mapping by direct combinatorial selection in cancer patients. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 46, pp. 18637-42, (2011) (PubMed).

Target Details for Annexin IV

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Alternative Name: ANXA4

Background: Annexin A4 (ANXA4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59 % identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells.
Alias Symbols: ANX4, PIG28, ZAP36
Protein Interaction Partner: PADI4, UBC, EZH2, DNAJB11, MPST, ANXA7, ANXA3, ANXA1, TINF2, POT1, SUMO2, NMT2, SMPD1, Cdk1, UCHL5, H2AFX, NFKB1, GP2,
Protein Size: 321

Molecular Weight: 35 kDa

Gene ID: 307

NCBI Accession: NM_001153, NP_001144

UniProt: Q6LES2