UBE2E3 antibody (N-Term)

Catalog No. ABIN2774921

This Rabbit Polyclonal antibody specifically detects UBE2E3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse and Rabbit.

Quick Overview for UBE2E3 antibody (N-Term) (ABIN2774921)

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2E3 Antibody

Binding Specificity: N-Term

Sequence: MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE2E3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2E3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2E3

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: UBE2E3

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Alias Symbols: UBCH9, UbcM2
Protein Interaction Partner: RNF185, MUL1, TRIM39, RNF5, MID1, RNF25, MARCH5, UBE2E3, UBA1, TARDBP, UBC, HTT, ITCH, HERC4, NEDD4L, WWP2, RNF14, RNF8, MDM2, BRCA1, BARD1, KLHL42, RCBTB1, CUL1, CUL3, CUL4A, SPOP, ATXN1, ARID3A, RCBTB2, MOT2, CNOT4, NFE2L2, MID2, TRIM38, TRIM27, TRIM37,
Protein Size: 207

Molecular Weight: 23 kDa

Gene ID: 10477

NCBI Accession: NM_006357, NP_006348

UniProt: Q969T4