WWP1 antibody (N-Term)

Catalog No. ABIN2774928

The Rabbit Polyclonal anti-WWP1 antibody has been validated for WB and IHC. It is suitable to detect WWP1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Pig.

Quick Overview for WWP1 antibody (N-Term) (ABIN2774928)

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-WWP1 Antibody

Binding Specificity: N-Term

Sequence: ATASPRSDTS NNHSGRLQLQ VTVSSAKLKR KKNWFGTAIY TEVVVDGEIT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against WWP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WWP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 922 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for WWP1

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Alternative Name: WWP1

Background: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. WWP1 is a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WWP1 belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes.WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants, however, the full length nature of these transcripts has not been defined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AIP5, DKFZp434D2111, Tiul1, hSDRP1
Protein Interaction Partner: SMAD3, FBXL18, WWP1, TRAF4, FAM189A2, UBE2L3, UBC, PTCH1, SMAD6, NOTCH1, EZH2, YWHAQ, CPSF6, TRAF6, LAPTM5, SMAD5, HSP90AA1, FBXL15, H2AFY2, PHF20L1, RNF11, ASH2L, PRKAA2, ATN1, LNX1, TP63, PTPN14, ERBB4, NFE2, SMAD7, SMAD4, SMAD2, SMAD1, RUNX2, Axin1, ga
Protein Size: 922

Molecular Weight: 105 kDa

Gene ID: 11059

NCBI Accession: NM_007013, NP_008944

UniProt: Q9H0M0