RAD54B antibody (Middle Region)

Catalog No. ABIN2775216

This anti-RAD54B antibody is a Rabbit Polyclonal antibody detecting RAD54B in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for RAD54B antibody (Middle Region) (ABIN2775216)

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAD54B Antibody

Binding Specificity: Middle Region

Sequence: NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against RAD54B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAD54B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 910 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD54B

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Alternative Name: RAD54B

Background: RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
Alias Symbols: FSBP, RDH54
Protein Interaction Partner: TCEANC, ATPAF2, ANKRD36BP1, ENKD1, MYO15B, GMCL1, RAD54B, CDC23, SUMO1, PRKAA1, FUS, HECW2, PSMA1, RAD51, NUDT18, NIF3L1, TCEB3, COPS6, SUMO2, UBC, WRN, ZNF580, LNX1, TRAPPC6A, RIBC2, CCDC33, UBE2I, GORASP2, DYRK2, DMC1, CSNK1E, CD2BP2,
Protein Size: 910

Molecular Weight: 103 kDa

Gene ID: 25788

NCBI Accession: NM_012415, NP_036547

UniProt: Q9Y620