DDX25 antibody (Middle Region)

Catalog No. ABIN2775217

This anti-DDX25 antibody is a Rabbit Polyclonal antibody detecting DDX25 in WB. Suitable for Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse and Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for DDX25 antibody (Middle Region) (ABIN2775217)

Target: DDX25 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 25 (DDX25))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX25 Antibody

Binding Specificity: Middle Region

Sequence: ALQTGRVVEQ MGKFCVDVQV MYAIRGNRIP RGTDITKQII IGTPGTVLDW

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against DDX25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DDX25

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 369 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX25 antibody (ABIN2775217)

"Why review?" in: Nature medicine, Vol. 13, Issue 8, pp. 887, (2007) (PubMed).

Target Details for DDX25

Target: DDX25 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 25 (DDX25))

Alternative Name: DDX25

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX25 is a member of this family. The protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.
Alias Symbols: GRTH
Protein Size: 369

Molecular Weight: 41 kDa

Gene ID: 29118

NCBI Accession: NM_013264, NP_037396

UniProt: Q9UHL0