Erythrocyte Ankyrin antibody (C-Term)

Catalog No. ABIN2775478

The Rabbit Polyclonal anti-Erythrocyte Ankyrin antibody has been validated for WB and IHC. It is suitable to detect Erythrocyte Ankyrin in samples from Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig and Cow.

Quick Overview for Erythrocyte Ankyrin antibody (C-Term) (ABIN2775478)

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Erythrocyte Ankyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Erythrocyte Ankyrin Antibody

Binding Specificity: C-Term

Sequence: VVRQIDLSSA DAAQEHEEVE LRGSGLQPDL IEGRKGAQIV KRASLKRGKQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ANK1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ANK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 155 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: ANK1

Background: Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats, a central region with a highly conserved spectrin binding domain, and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
Alias Symbols: ANK, SPH1, SPH2
Protein Interaction Partner: UBC, SPTA1, SUMO1, CD44, TGFBR1, SPTBN1, KCTD6, OBSCN, CUL3, NEDD8, SPTB, BLOC1S6, NFASC, NBPF3, TIAM1, L1CAM, TTN, SPTAN1, RHAG, EPB42, ITPR3, SLC4A1, SLC4A3,
Protein Size: 155

Molecular Weight: 18 kDa

Gene ID: 286

NCBI Accession: NM_020478, NP_065211

UniProt: Q53ER1

Pathways: Synaptic Membrane