GNAS antibody (N-Term)

Catalog No. ABIN2775542

The Rabbit Polyclonal anti-GNAS antibody has been validated for WB and IHC. It is suitable to detect GNAS in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Horse and Guinea Pig. There are 2+ publications available.

Quick Overview for GNAS antibody (N-Term) (ABIN2775542)

Target: GNAS (GNAS Complex Locus (GNAS))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GNAS Antibody

Binding Specificity: N-Term

Sequence: VYRATHRLLL LGAGESGKST IVKQMRILHV NGFNGEGGEE DPQAARSNSD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GNAS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GNAS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 394 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GNAS antibody (ABIN2775542)

Moreno-Smith, Lee, Lu, Nagaraja, He, Rupaimoole, Han, Jennings, Roh, Nishimura, Kang, Allen, Armaiz, Matsuo, Shahzad, Bottsford-Miller, Langley, Cole, Lutgendorf, Siddik, Sood: "Biologic effects of dopamine on tumor vasculature in ovarian carcinoma." in: Neoplasia (New York, N.Y.), Vol. 15, Issue 5, pp. 502-10, (2013) (PubMed).

Masyuk, Huang, Radtke, Gajdos, Splinter, Masyuk, Gradilone, LaRusso: "Ciliary subcellular localization of TGR5 determines the cholangiocyte functional response to bile acid signaling." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 304, Issue 11, pp. G1013-24, (2013) (PubMed).

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS

Background: Mutations in GNAS gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Alias Symbols: AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, NESP, PHP1C
Protein Interaction Partner: PANX1, AXIN1, UBC, FUS, OPTN, PTGIR, HLA-A, ADRB2, NUCB2, NUCB1, LAMTOR1, SLC25A12, GNAQ, GNA11, UBD, TBXA2R, GNB1, AVPR2, SUMO1, PCK1, Ric8b, GNG2, CALM1, Haus1, Trim69, Cbx1, RIC8A, TTC1, SNX13, ADCY5, CRHR1, PTGDR, TSHR, CAV3, HTR6, RGS2, ADCY6, VIPR1,
Protein Size: 394

Molecular Weight: 46 kDa

Gene ID: 2778

NCBI Accession: NM_000516, NP_000507

UniProt: P63092

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis