ACLY antibody (Middle Region)

Catalog No. ABIN2775558

Product Details anti-ACLY Antibody

Target: ACLY (ATP Citrate Lyase (ACLY))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Zebrafish (Danio rerio), Rabbit, Sheep, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACLY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LRSAYDSTME TMNYAQIRTI AIIAEGIPEA LTRKLIKKAD QKGVTIIGPA

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%, Sheep: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ACLY. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ACLY

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1101 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACLY antibody (ABIN2775558)

Infantino, Iacobazzi, Palmieri, Menga: "ATP-citrate lyase is essential for macrophage inflammatory response." in: Biochemical and biophysical research communications, Vol. 440, Issue 1, pp. 105-11, (2013) (PubMed).

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY (ACLY Products)

Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acety
Alias Symbols: ACL, ATPCL, CLATP
Protein Interaction Partner: UBC, UBR4, SIRT2, HUWE1, ISG15, KAT2B, SUMO2, SUMO3, STAU1, SUMO1, NEDD8, MDM2, ASB15, WWOX, TRIM25, MAGED2, BAG3, PPP6C, NCL, MCM6, MCM2, KPNA4, KPNA2, PRMT1, CDKN2A, SRRT, UBD, FN1, APP, SHC1, CUL3, CAND1, CUL2, SIRT7, UL27, HDAC5, TERF2, TERF1, GSK3B,
Protein Size: 1101

Molecular Weight: 121 kDa

Gene ID: 47

NCBI Accession: NM_001096, NP_001087

UniProt: P53396

Pathways: Warburg Effect