COX IV antibody (N-Term)

Catalog No. ABIN2775574

The Rabbit Polyclonal anti-COX IV antibody has been validated for WB and IHC. It is suitable to detect COX IV in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse and Rabbit. There are 3+ publications available.

Quick Overview for COX IV antibody (N-Term) (ABIN2775574)

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-COX IV Antibody

Binding Specificity: N-Term

Sequence: AISTSVCVRA HESVVKSEDF SLPAYMDRRD HPLPEVAHVK HLSASQKALK

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 85%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against COX4I1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX4I1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 169 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COX IV antibody (ABIN2775574)

Hwang, Dornbos, Steidemann, Dunivin, Rizzo, LaPres: "Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome." in: Toxicology and applied pharmacology, (2016) (PubMed).

Geerling, Boon, van der Zon, van den Berg, van den Hoek, Lombès, Princen, Havekes, Rensen, Guigas: "Metformin lowers plasma triglycerides by promoting VLDL-triglyceride clearance by brown adipose tissue in mice." in: Diabetes, Vol. 63, Issue 3, pp. 880-91, (2014) (PubMed).

Snyder, Wang, Patton, Arosio, Levi, Earley, Allen, Connor: "Mitochondrial ferritin in the substantia nigra in restless legs syndrome." in: Journal of neuropathology and experimental neurology, Vol. 68, Issue 11, pp. 1193-9, (2009) (PubMed).

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX4I1

Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 is the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
Alias Symbols: COX4, COXIV, MGC72016, COX4-1
Protein Interaction Partner: SDCBP, COX1, env, APP, UBC, ICT1, TMBIM4, NELFCD,
Protein Size: 169

Molecular Weight: 19 kDa

Gene ID: 1327

NCBI Accession: NM_001861, NP_001852

UniProt: P13073

Pathways: Proton Transport