RFWD2 antibody (Middle Region)

Catalog No. ABIN2775650

The Rabbit Polyclonal anti-RFWD2 antibody (ABIN2775650) specifically detects RFWD2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse samples.

Quick Overview for RFWD2 antibody (Middle Region) (ABIN2775650)

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFWD2 Antibody

Binding Specificity: Middle Region

Sequence: MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Rfwd2. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 733 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFWD2

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Alternative Name: Rfwd2

Background: Rfwd2 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It is involved in JUN ubiquitination and degradation and directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Rfwd2 probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Alias Symbols: AI316802, C80879, Cop1
Protein Interaction Partner: CRY2, Reg1, Ptpn11, Fasn, Trib3, Trib2, Trib1,
Protein Size: 733

Molecular Weight: 80 kDa

Gene ID: 26374

NCBI Accession: NM_011931, NP_036061

UniProt: Q9R1A8

Pathways: Photoperiodism