UBE2D1 antibody (Middle Region)

Catalog No. ABIN2775671

The Rabbit Polyclonal anti-UBE2D1 antibody has been validated for WB. It is suitable to detect UBE2D1 in samples from Human, Mouse, Rat, Dog, Cow, Goat, Horse, Pig, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for UBE2D1 antibody (Middle Region) (ABIN2775671)

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Goat, Horse, Pig, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2D1 Antibody

Binding Specificity: Middle Region

Sequence: SQWSPALTVS KVLLSICSLL CDPNPDDPLV PDIAQIYKSD KEKYNRHARE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE2D1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2D1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 147 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2D1

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Alternative Name: UBE2D1

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Alias Symbols: E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Protein Interaction Partner: RBX1, STUB1, RNF7, RNF14, MID1, MDM2, EP300, CREBBP, ANAPC11, DTL, RNF115, MKRN2, NEDD4L, FAF2, TRIM32, MID2, RNF111, RNF5, RSP5, GPB1, NHLRC1, RFFL, UHRF2, DTX2, ZNRF1, TRIM63, RNF146, FBXO31, RNF26, TRIM39, RNF126, FBXW7, NEDD4, CUL1, CUL3, MKRN3, UBE3A
Protein Size: 147

Molecular Weight: 16 kDa

Gene ID: 7321

NCBI Accession: NM_001204880, NP_003329

UniProt: P51668

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades