UBE2I antibody (Middle Region)

Catalog No. ABIN2775681

The Rabbit Polyclonal anti-UBE2I antibody has been validated for WB. It is suitable to detect UBE2I in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Rabbit, Horse, Guinea Pig and Saccharomyces cerevisiae.

Quick Overview for UBE2I antibody (Middle Region) (ABIN2775681)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Rabbit, Horse, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2I Antibody

Binding Specificity: Middle Region

Sequence: KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2I. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2I

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 474 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.
Alias Symbols: C358B7.1, P18, UBC9
Protein Interaction Partner: TRIM21, SP100, SUMO2, RANGAP1, PTEN, LMO2, PIAS2, TRIM23, PARP1, PIAS4, UBXN1, USP25, SETBP1, SIRT1, PUF60, HMGA1, GOLGA2, FHL3, ELK1, DES, DAXX, CREBBP, CLK2, RCBTB2, CEBPD, CBS, CAPN2, PPARGC1A, RFPL3, TRIM38, IKZF1, RNF40, XBP1, KRT19, HNRNPC, ZBTB8A,
Protein Size: 474

Molecular Weight: 52 kDa

Gene ID: 7329

NCBI Accession: NM_018045, NP_003336

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway