CIAO1 antibody (Middle Region)

Catalog No. ABIN2775776

The Rabbit Polyclonal anti-CIAO1 antibody has been validated for WB and IHC. It is suitable to detect CIAO1 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for CIAO1 antibody (Middle Region) (ABIN2775776)

Target: CIAO1 (Cytosolic Iron-Sulfur Protein Assembly 1 (CIAO1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIAO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CIAO1 Antibody

Binding Specificity: Middle Region

Sequence: SVLNSHTQDV KHVVWHPSQE LLASASYDDT VKLYREEEDD WVCCATLEGH

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%

Characteristics: This is a rabbit polyclonal antibody against CIAO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CIAO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 339 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CIAO1 antibody (ABIN2775776)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for CIAO1

Target: CIAO1 (Cytosolic Iron-Sulfur Protein Assembly 1 (CIAO1))

Alternative Name: CIAO1

Background: CIAO1 belongs to the WD repeat CIA1 family. It seems to specifically modulate the transactivation activity of WT1.
Alias Symbols: CIA1, WDR39
Protein Interaction Partner: FAM96B, SF1, MEOX1, FAM96A, MMS19, FMNL2, TOE1, DPP9, FMNL3, CTU1, PPIL4, MAK16, WDR75, NSRP1, EEPD1, WDR61, CDC73, UPF3B, NARFL, YAE1D1, TYW1, ELP2, ELP3, UCKL1, CDKAL1, DPP8, ELP6, PAF1, PLEKHA5, PHAX, RTEL1, ELP4, POLA2, ELP5, WDR43, ERP29, GLRX3, CTR9
Protein Size: 339

Molecular Weight: 38 kDa

Gene ID: 9391

NCBI Accession: NM_004804, NP_004795

UniProt: O76071