FHL2 antibody (C-Term)

Catalog No. ABIN2775839

This Rabbit Polyclonal antibody specifically detects FHL2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) and has been mentioned in 3+ publications.

Quick Overview for FHL2 antibody (C-Term) (ABIN2775839)

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FHL2 Antibody

Binding Specificity: C-Term

Sequence: RFTARDDFAY CLNCFCDLYA KKCAGCTNPI SGLGGTKYIS FEERQWHNDC

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 87%

Characteristics: This is a rabbit polyclonal antibody against FHL2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FHL2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 279 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FHL2 antibody (ABIN2775839)

Han, Wu, Zhao, Meng, Si, Yang, Fu, Yu: "FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells." in: Nucleic acids research, Vol. 37, Issue 12, pp. 3996-4009, (2009) (PubMed).

Saeki, Endo, Ide, Nagashima, Yumoto, Toyoda, Suzuki, Hayashizaki, Sakaki, Okada-Hatakeyama: "Ligand-specific sequential regulation of transcription factors for differentiation of MCF-7 cells." in: BMC genomics, Vol. 10, pp. 545, (2009) (PubMed).

Lee, Chung, Kang: "Combined treatment of high pressure and heat on killing spores of Alicyclobacillus acidoterrestris in apple juice concentrate." in: Journal of food protection, Vol. 69, Issue 5, pp. 1056-60, (2006) (PubMed).

Target Details for FHL2

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Alternative Name: FHL2

Background: FHL2 is a member of LIM proteins that contain a highly conserved double zinc finger motif called the LIM domain.
Alias Symbols: DRAL, AAG11, FHL-2, SLIM3, SLIM-3
Protein Interaction Partner: SP2, RFX3, REL, NRF1, JUP, INCA1, FAM154A, ZNF417, FAM129A, GLYR1, CCDC92, ARHGAP9, KIAA1217, GNG12, DCP1A, ZFP64, BANP, KANK2, RAI2, ZMYM4, BLZF1, ZNF131, CSN2, SIGLEC6, DTX2, TRIM63, TRIM55, FOXO1, SUMO2, PTCH1, DCTN3, HEXIM1, ERP44, SMAD4, SMAD3, SMAD2
Protein Size: 279

Molecular Weight: 32 kDa

Gene ID: 2274

NCBI Accession: NM_201555, NP_963849

UniProt: Q14192

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha