KLHL3 antibody (N-Term)

Catalog No. ABIN2775874

This Rabbit Polyclonal antibody specifically detects KLHL3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Dog, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for KLHL3 antibody (N-Term) (ABIN2775874)

Target: KLHL3 (Kelch-Like 3 (KLHL3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KLHL3 Antibody

Binding Specificity: N-Term

Sequence: CSPYFCAMFT GDMSESKAKK IEIKDVDGQT LSKLIDYIYT AEIEVTEENV

Predicted Reactivity: Cow: 100%, Dog: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against KLHL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KLHL3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 587 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KLHL3

Target: KLHL3 (Kelch-Like 3 (KLHL3))

Alternative Name: KLHL3

Background: KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77 % similarity with Drosophila kelch and 89 % similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q, however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
Alias Symbols: FLJ40871, KIAA1129, MGC44594, PHA2D
Protein Interaction Partner: WNK4, KLHL12, KLHL3, KEAP1, MORF4L2, CUL3, UBC, C6orf165, WNK3, WNK2, WNK1, RBX1, SLC12A3, ELAVL1, SYNCRIP, SYT2, OPRD1, GNAO1, GNAI1,
Protein Size: 587

Molecular Weight: 65 kDa

Gene ID: 26249

NCBI Accession: NM_017415, NP_059111

UniProt: Q9UH77