Angiomotin antibody (N-Term)

Catalog No. ABIN2775937

This anti-Angiomotin antibody is a Rabbit Polyclonal antibody detecting Angiomotin in WB and IHC. Suitable for Human, Mouse, Rat, Horse, Dog, Guinea Pig, Rabbit, Cow and Zebrafish (Danio rerio).

Quick Overview for Angiomotin antibody (N-Term) (ABIN2775937)

Target: Angiomotin (AMOT)

Reactivity: Human, Mouse, Rat, Horse, Dog, Guinea Pig, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiomotin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Angiomotin Antibody

Binding Specificity: N-Term

Sequence: TANKQLAEKE YEGSEDTRKT ISQLFAKNKE SQREKEKLEA ELATARSTNE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against AMOT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AMOT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 675 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Angiomotin

Target: Angiomotin (AMOT)

Alternative Name: AMOT

Background: AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: KIAA1071
Protein Interaction Partner: AMOT, C1orf216, WDYHV1, MED4, SNAPC5, PPP2R4, LURAP1, UNC119, LMO4, PIN1, MAGEA4, KRT31, KRT15, FUS, SEPT1, CEP76, HAUS2, LATS2, CEP250, CDKN1A, ACTB, ACTA1, YAP1, SART3, AURKB, MAPK3, BAG3, WWOX, ITCH, LATS1, YWHAG, UBC, PARK2, AMOTL1, SPICE1, MPP7, CNTR
Protein Size: 675

Molecular Weight: 73 kDa

Gene ID: 154796

NCBI Accession: NM_133265, NP_573572

UniProt: Q4VCS5

Pathways: Cell-Cell Junction Organization, Regulation of Cell Size