NFX1 antibody (N-Term)

Catalog No. ABIN2776010

This anti-NFX1 antibody is a Rabbit Polyclonal antibody detecting NFX1 in WB. Suitable for Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse and Pig.

Quick Overview for NFX1 antibody (N-Term) (ABIN2776010)

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFX1 Antibody

Binding Specificity: N-Term

Sequence: MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1024 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Alternative Name: NFX1

Background: NFX1 binds to the X-box motif of MHC class II genes and represses their expression. NFX1 may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. NFX1 mediates E2-dependent ubiquitination.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Alias Symbols: DKFZp779G2416, MGC20369, NFX2
Protein Interaction Partner: UBC, BMI1, HECW2, SRSF4, SRSF3, RPS19, RPS3A, RPL22, RPL17, RPL13, RPL7A, RPL6, RPL3, PABPC3, YBX1, NCL, HNRNPM, HSPA1A, HNRNPU, HNRNPH1, HNRNPA2B1, HIST1H2AE, EEF1A2, DMD, DDX5, FAM208B, PABPC1, RPL13A, LARP1, APC2, MATR3, HIST1H4E, USP9X, SIN3A, MAML3,
Protein Size: 1024

Molecular Weight: 113 kDa

Gene ID: 4799

NCBI Accession: NM_147133, NP_667344