DIDO1 antibody (N-Term)

Catalog No. ABIN2776068

This Rabbit Polyclonal antibody specifically detects DIDO1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Saccharomyces cerevisiae.

Quick Overview for DIDO1 antibody (N-Term) (ABIN2776068)

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIDO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DIDO1 Antibody

Binding Specificity: N-Term

Sequence: ERVEQFLTIA RRRGRRSMPV SLEDSGEPTS CPATDAETAS EGSVESASET

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 86%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%, Yeast: 79%

Characteristics: This is a rabbit polyclonal antibody against DIDO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DIDO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 544 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DIDO1

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Alternative Name: DIDO1

Background: In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. DIDO1 gene is similar to the mouse gene and therefore is thought to be involved in apoptosis.Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.
Alias Symbols: BYE1, DIO1, DATF1, DIDO2, DIDO3, DIO-1, DATF-1, C20orf158, dJ885L7.8
Protein Interaction Partner: UBC, WWOX, RPA3, RPA2, RPA1, EED, BMI1, HECW2, SRPK2, KCNK4, ARAF, Dlg4, H2AFY, U2AF1, SON, RPL18A, KRT7, APP, PCDHA2, H2AFY2, GNL3, RRS1, SIRT7, SUMO2, WWP2, WWP1, DVL3, SRSF1, HNRNPK,
Protein Size: 544

Molecular Weight: 59 kDa

Gene ID: 11083

NCBI Accession: NM_080797, NP_542987

Pathways: Hormone Activity