TRPM4 antibody (N-Term)

Catalog No. ABIN2776221

There is 1 publication for this product available. The Rabbit Polyclonal anti-TRPM4 antibody is suitable to detect TRPM4 in samples from Human, Rat, Mouse, Cow, Dog, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for TRPM4 antibody (N-Term) (ABIN2776221)

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRPM4 Antibody

Binding Specificity: N-Term

Sequence: ELLTVYSSED GSEEFETIVL KALVKACGSS EASAYLDELR LAVAWNRVDI

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRPM4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRPM4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1214 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRPM4 antibody (ABIN2776221)

Kuras, Yun, Chimote, Neumeier, Conforti: "KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e43859, (2012) (PubMed).

Target Details for TRPM4

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Alternative Name: TRPM4

Background: TRPM4 is a calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca2+, it is impermeable to it. TRPM4 mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. TRPM4 is involved in myogenic constriction of cerebral arteries. It controls insulin secretion in pancreatic beta-cells. TRPM4 may also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca2+ overload.
Alias Symbols: FLJ20041, TRPM4B, PFHB1B
Protein Interaction Partner: FUS, UBC, TRPM4,
Protein Size: 1214

Molecular Weight: 134 kDa

Gene ID: 54795

NCBI Accession: NM_017636, NP_060106

UniProt: Q8TD43

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response