Bestrophin 4 antibody (N-Term)

Catalog No. ABIN183172

This Rabbit Polyclonal antibody specifically detects Bestrophin 4 in WB. It exhibits reactivity toward Human, Dog, Cow, Rabbit, Guinea Pig, Mouse, Horse, Rat and Zebrafish (Danio rerio) and has been mentioned in 2+ publications.

Quick Overview for Bestrophin 4 antibody (N-Term) (ABIN183172)

Target: Bestrophin 4 (BEST4)

Reactivity: Human, Dog, Cow, Rabbit, Guinea Pig, Mouse, Horse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bestrophin 4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Bestrophin 4 Antibody

Binding Specificity: N-Term

Sequence: MTVSYTLKVA EARFGGFSGL LLRWRGSIYK LLYKEFLLFG ALYAVLSITY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 79%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against VMD2L2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VMD2L2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 473 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Bestrophin 4 antibody (ABIN183172)

Milenkovic, Soria, Aldehni, Schreiber, Kunzelmann: "Functional assembly and purinergic activation of bestrophins." in: Pflügers Archiv : European journal of physiology, Vol. 458, Issue 2, pp. 431-41, (2009) (PubMed).

Stöhr, Marquardt, Nanda, Schmid, Weber: "Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family." in: European journal of human genetics : EJHG, Vol. 10, Issue 4, pp. 281-4, (2002) (PubMed).

Target Details for Bestrophin 4

Target: Bestrophin 4 (BEST4)

Alternative Name: VMD2L2

Background: VMD2L2 is 1 of 3 VMD2-like genes which encode transmembrane spanning proteins that share a homology region with a high content of aromatic residues including an invariant arginine (R), phenylalanine (F), and proline (P) motif.
Alias Symbols: VMD2L2
Protein Interaction Partner: Dlg4,
Protein Size: 473

Molecular Weight: 52 kDa

Gene ID: 266675

NCBI Accession: NM_153274, NP_695006

UniProt: Q8NFU0