Bestrophin 4 antibody (BEST4) (Internal Region)

Details for Product anti-BEST4 Antibody No. ABIN1048355
Target
Binding Specificity
Internal Region
3
3
2
Reactivity
Human, Monkey
26
4
3
3
3
2
2
2
1
1
1
1
1
Host
Rabbit
26
Clonality
Polyclonal
Conjugate
This Bestrophin 4 antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
13
10
6
4
3
2
Options
Brand IHC-plus™
Immunogen Synthetic 15 amino acid peptide from internal region of human BEST4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Rabbit (93%), Hamster (87%), Pig (80%).

Type of Immunogen: Synthetic peptide
Specificity Human BEST4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Rabbit (93%) Hamster (87%) Pig (80%).
Purification Immunoaffinity purified
Target
Alternative Name BEST4 (BEST4 Antibody Abstract)
Background Name/Gene ID: BEST4
Subfamily: Bestrophin
Family: Ion channel

Synonyms: BEST4, Bestrophin-4, Bestrophin 4, VMD2L2
Gene ID 266675
Application Notes Approved: IHC, IHC-P (2.5 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Images
Image no. 1 for anti-Bestrophin 4 (BEST4) (Internal Region) antibody (ABIN1048355) Anti-BEST4 antibody IHC staining of human stomach. Immunohistochemistry of formalin-f...
Image no. 2 for anti-Bestrophin 4 (BEST4) (Internal Region) antibody (ABIN1048355) Anti-BEST4 antibody IHC staining of human small intestine. Immunohistochemistry of fo...