MED21 antibody (N-Term)

Catalog No. ABIN2776418

The Rabbit Polyclonal anti-MED21 antibody has been validated for WB and ChIP. It is suitable to detect MED21 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for MED21 antibody (N-Term) (ABIN2776418)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED21 Antibody

Binding Specificity: N-Term

Sequence: FCNAIGVLQQ CGPPASFSNI QTAINKDQPA NPTEEYAQLF AALIARTAKD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against Med21. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 144 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: Med21

Background: Med21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: 0610007L03Rik, AI449604, D19234, D6Ertd782e, Srb7, Surb7
Protein Interaction Partner: Med21, Med6, Aagab, Med7, Mcrs1, Pik3ca, Ltb, Brd2, Nfe2,
Protein Size: 144

Molecular Weight: 15 kDa

Gene ID: 108098

NCBI Accession: NM_025315, NP_079591

UniProt: Q9CQ39

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha