BOLL antibody (N-Term)

Catalog No. ABIN2776601

The Rabbit Polyclonal anti-BOLL antibody is suitable to detect BOLL in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for BOLL antibody (N-Term) (ABIN2776601)

Target: BOLL (Bol, Boule-Like (BOLL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BOLL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BOLL Antibody

Binding Specificity: N-Term

Sequence: GGIDFKTNES DLRKFFSQYG SVKEVKIVND RAGVSKGYGF VTFETQEDAQ

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against BOLL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BOLL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 283 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BOLL

Target: BOLL (Bol, Boule-Like (BOLL))

Alternative Name: BOLL

Background: This gene belongs to the DAZ gene family required for germ cell development. BOLL is an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: BOULE
Protein Interaction Partner: ZNF385C, HNRNPLL, C1orf94, RBFOX2, RBPMS, TFG, SERF2, NPBWR2, DAZ1, DAZ4,
Protein Size: 283

Molecular Weight: 31 kDa

Gene ID: 66037

NCBI Accession: NM_033030, NP_149019

UniProt: Q8N9W6