A1CF antibody (N-Term)

Catalog No. ABIN2776627

This anti-A1CF antibody is a Rabbit Polyclonal antibody detecting A1CF in WB. Suitable for Human.

Quick Overview for A1CF antibody (N-Term) (ABIN2776627)

Target: A1CF (APOBEC1 Complementation Factor (A1CF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This A1CF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-A1CF Antibody

Binding Specificity: N-Term

Sequence: EAVCLGTCPE PEASMSTAIP GLKKGNNALQ SIILQTLLEK ENGQRKYGGP

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against A1CF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human A1CF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 594 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for A1CF

Target: A1CF (APOBEC1 Complementation Factor (A1CF))

Alternative Name: A1CF

Background: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. A1CF has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined.Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined.
Alias Symbols: ACF, ACF64, ACF65, APOBEC1CF, ASP, MGC163391, RP11-564C4.2
Protein Interaction Partner: SDPR, TRAF1, REL, FHL3, KHSRP, SYNCRIP, TNPO2, CELF2, APOBEC1,
Protein Size: 594

Molecular Weight: 65 kDa

Gene ID: 29974

NCBI Accession: NM_138933, NP_620311

UniProt: Q9NQ94