FZD9 antibody (N-Term)

Catalog No. ABIN2776709

This Rabbit Polyclonal antibody specifically detects FZD9 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for FZD9 antibody (N-Term) (ABIN2776709)

Target: FZD9 (Frizzled Family Receptor 9 (FZD9))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FZD9 Antibody

Binding Specificity: N-Term

Sequence: RPPGDLGPGA GGSGTCENPE KFQYVEKSRS CAPRCGPGVE VFWSRRDKDF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 92%, Rat: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against FZD9. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FZD9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 591 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD9 antibody (ABIN2776709)

Tennis, Van Scoyk, Heasley, Vandervest, Weiser-Evans, Freeman, Keith, Simpson, Nemenoff, Winn: "Prostacyclin inhibits non-small cell lung cancer growth by a frizzled 9-dependent pathway that is blocked by secreted frizzled-related protein 1." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 3, pp. 244-53, (2010) (PubMed).

Target Details for FZD9

Target: FZD9 (Frizzled Family Receptor 9 (FZD9))

Alternative Name: FZD9

Background: FZD9 contains 1 FZ (frizzled) domain and belongs to the G-protein coupled receptor Fz/Smo family. It is receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members. It may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.
Alias Symbols: FZD3, CD349
Protein Interaction Partner: KRTAP10-3, SIAH1, MDFI, WNT7A, WNT1, WNT2,
Protein Size: 591

Molecular Weight: 65 kDa

Gene ID: 8326

NCBI Accession: NM_003508, NP_003499

UniProt: O00144

Pathways: WNT Signaling