FZD8 antibody (N-Term)

Catalog No. ABIN2776728

This anti-FZD8 antibody is a Rabbit Polyclonal antibody detecting FZD8 in WB. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 2+ publications.

Quick Overview for FZD8 antibody (N-Term) (ABIN2776728)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FZD8 Antibody

Binding Specificity: N-Term

Sequence: PDTLCMDYNR TDLTTAAPSP PRRLPPPPPG EQPPSGSGHG RPPGARPPHR

Predicted Reactivity: Dog: 100%, Human: 100%, Mouse: 93%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against FZD8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FZD8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 694 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD8 antibody (ABIN2776728)

Xu, Wen, Liu, Xu, Yang, Liu, Feng, An: "MicroRNA-375 suppresses human colorectal cancer metastasis by targeting Frizzled 8." in: Oncotarget, Vol. 7, Issue 26, pp. 40644-40656, (2016) (PubMed).

Yin, Xu, Bonfil, Banerjee, Sarkar, Sethi, Reddy: "Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 12, Issue 4, pp. 491-8, (2013) (PubMed).

Target Details for FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: FZD8

Background: FZD8 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
Alias Symbols: FZ-8, hFZ8
Protein Interaction Partner: WNT1, LRP5, LRP6, SUMO1, GOPC, RYK,
Protein Size: 694

Molecular Weight: 73 kDa

Gene ID: 8325

NCBI Accession: NM_031866, NP_114072

UniProt: Q9H461

Pathways: WNT Signaling