Doublecortin antibody (C-Term)

Catalog No. ABIN2776749

The Rabbit Polyclonal anti-Doublecortin antibody has been validated for WB. It is suitable to detect Doublecortin in samples from Human, Mouse, Pig, Horse and Dog.

Quick Overview for Doublecortin antibody (C-Term) (ABIN2776749)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse, Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Doublecortin Antibody

Binding Specificity: C-Term

Sequence: TAGPKASPTP QKTSAKSPGP MRRSKSPADS ANGTSSSQLS TPKSKQSPIS

Predicted Reactivity: Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%

Characteristics: This is a rabbit polyclonal antibody against DCX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DCX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX

Background: In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The protein contains two doublecortin domains, which bind microtubules. In addition, DCX interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex.In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.
Alias Symbols: DBCN, DC, LISX, SCLH, XLIS
Protein Interaction Partner: KIFC3, GOLGA2, TRIM23, KRTAP10-8, KRT40, RINT1, MID2, SPAG5, IKZF1, CALCOCO2, ZBTB5, TRIM27, MEOX1, FBXO25, APP, PPP1R9B, PPP1CA, PAFAH1B1, ACD, TINF2, POT1, TERF1, SPP1, USP9X, NFASC, TRIM39, AP1M1, AP2M1, DCX, CDK5,
Protein Size: 441

Molecular Weight: 49 kDa

Gene ID: 1641

NCBI Accession: NM_000555, NP_000546

UniProt: O43602