Tropomyosin antibody (N-Term)

Catalog No. ABIN2776783

This anti-Tropomyosin antibody is a Rabbit Polyclonal antibody detecting Tropomyosin in WB and IHC. Suitable for Human, Rat, Mouse, Zebrafish (Danio rerio), Rabbit, Cow, Dog, Horse, Guinea Pig and Sheep.

Quick Overview for Tropomyosin antibody (N-Term) (ABIN2776783)

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Rabbit, Cow, Dog, Horse, Guinea Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tropomyosin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tropomyosin Antibody

Binding Specificity: N-Term

Sequence: DRAEQAEADK KAAEDRSKQL EDELVSLQKK LKGTEDELDK YSEALKDAQE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TPM1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TPM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 284 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Tropomyosin

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Alternative Name: TPM1

Background: TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
Alias Symbols: CMH3, TMSA, TPM1-alpha, CMD1Y, C15orf13, HTM-alpha
Protein Interaction Partner: GOLGA2, CAGE1, C1orf216, SYCE1, KXD1, TFPT, MAD1L1, TPM4, TP53, TNNT1, UBC, MDM2, ETFA, FOXK1, KIAA1598, PPP6R3, APPL2, CDC37, MGEA5, STUB1, ABI2, DNAJC7, HSP90B1, PARK2, LRCH3, PAN2, TGFBR1, SPTBN1, ATF2, MDC1, BRCA1, PPP4R2, SRXN1, NUDCD2, RBM15, THOC2,
Protein Size: 284

Molecular Weight: 31 kDa

Gene ID: 7168

NCBI Accession: NM_000366, NP_000357

UniProt: P09493

Pathways: Regulation of Actin Filament Polymerization