ACTN2 antibody (N-Term)

Catalog No. ABIN2776829

This Rabbit Polyclonal antibody specifically detects ACTN2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbitand has been mentioned in 1 publication.

Quick Overview for ACTN2 antibody (N-Term) (ABIN2776829)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACTN2 Antibody

Binding Specificity: N-Term

Sequence: NQIEPGVQYN YVYDEDEYMI QEEEWDRDLL LDPAWEKQQR KTFTAWCNSH

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ACTN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 894 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACTN2 antibody (ABIN2776829)

Yan, Wang, Sharova, Sharov, Ling, Piao, Aiba, Matoba, Wang, Ko: "BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1155-65, (2008) (PubMed).

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background: The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-39 DC378038.1 17-55 40-2961 BC051770.1 1-2922 2962-3458 CB153006.1 65-561 3459-3637 AL359185.25 28531-28709 3638-4210 M86406.1 3609-4181 4211-4528 AL359185.25 29283-29600
Alias Symbols: CMD1AA
Protein Interaction Partner: ACTN2, FAM154A, BRMS1L, MICALL2, MYOZ2, PDLIM3, SP100, SNAI1, MOS, RTP5, SYNPO2, SUMO1, IGSF8, ERBB2IP, CAPN1, ZNF446, RPL35, UBC, NOS3, ACTN1, ACTN4, FBXL22, COIL, SUMO3, ATXN2, KCNA5, LDB3, KCNN2, PSMA3, ITGB3BP, SNW1, MED14, NR1I2, KAT2B, NRIP1, ATXN7,
Protein Size: 894

Molecular Weight: 104 kDa

Gene ID: 88

NCBI Accession: NM_001103, NP_001094

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity