ACTN2 antibody

Catalog No. ABIN2855582

Product Details anti-ACTN2 Antibody

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Based on sequence homology, this antibody may cross-react with other Actinin family members.

Cross-Reactivity: Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to alpha Actinin 2 (actinin, alpha 2)
alpha Actinin 2 antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.18 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ACTN2 antibody (ABIN2855582)

Roberts, Romero, Mobley, Mumford, Roberson, Haun, Vann, Osburn, Holmes, Greer, Lockwood, Parry, Kavazis: "Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training." in: PeerJ, Vol. 6, pp. e5338, (2018) (PubMed).

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: actinin alpha 2 (ACTN2 Products)

Synonyms: MGC89234 antibody, ACTN2 antibody, 1110008F24Rik antibody, CMD1AA antibody, wu:fd44c03 antibody, zgc:123223 antibody, actinin alpha 2 antibody, actinin, alpha 2b antibody, actinin alpha 2 S homeolog antibody, actn2 antibody, ACTN2 antibody, Actn2 antibody, actn2b antibody, actn2.S antibody

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

Cellular Localization: Cytoplasm , myofibril , sarcomere , Z-disk

Molecular Weight: 104 kDa

Gene ID: 88

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity