HMGCS2 antibody (N-Term)

Catalog No. ABIN2776880

There are 2+ publications for this product available. The Rabbit Polyclonal anti-HMGCS2 antibody is suitable to detect HMGCS2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Goat, Horse and Cow. It has been validated for WB.

Quick Overview for HMGCS2 antibody (N-Term) (ABIN2776880)

Target: HMGCS2 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial) (HMGCS2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Goat, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGCS2 Antibody

Binding Specificity: N-Term

Sequence: PKDVGILALE VYFPAQYVDQ TDLEKYNNVE AGKYTVGLGQ TRMGFCSVQE

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HMGCS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HMGCS2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 508 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGCS2 antibody (ABIN2776880)

Clara, Schumacher, Ramachandran, Fedele, Krieger, Langhans, Mansouri: "Metabolic Adaptation of the Small Intestine to Short- and Medium-Term High-Fat Diet Exposure." in: Journal of cellular physiology, Vol. 232, Issue 1, pp. 167-75, (2016) (PubMed).

Karimian Azari, Leitner, Jaggi, Langhans, Mansouri: "Possible role of intestinal fatty acid oxidation in the eating-inhibitory effect of the PPAR-? agonist Wy-14643 in high-fat diet fed rats." in: PLoS ONE, Vol. 8, Issue 9, pp. e74869, (2013) (PubMed).

Target Details for HMGCS2

Target: HMGCS2 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial) (HMGCS2))

Alternative Name: HMGCS2

Background: HMGCS2 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Protein Interaction Partner: UBC, APP, YWHAQ,
Protein Size: 508

Molecular Weight: 56 kDa

Gene ID: 3158

NCBI Accession: NM_005518, NP_005509

UniProt: P54868

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha