HMGCS2 antibody (N-Term)

Catalog No. ABIN2776880

The Rabbit Polyclonal anti-HMGCS2 antibody has been validated for WB. It is suitable to detect HMGCS2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Goat, Horse and Cow. There are 2+ publications available.

Quick Overview for HMGCS2 antibody (N-Term) (ABIN2776880)

Target: HMGCS2 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial) (HMGCS2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Goat, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGCS2 Antibody

Binding Specificity: N-Term

Sequence: PKDVGILALE VYFPAQYVDQ TDLEKYNNVE AGKYTVGLGQ TRMGFCSVQE

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HMGCS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HMGCS2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 508 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGCS2 antibody (ABIN2776880)

Clara, Schumacher, Ramachandran, Fedele, Krieger, Langhans, Mansouri: "Metabolic Adaptation of the Small Intestine to Short- and Medium-Term High-Fat Diet Exposure." in: Journal of cellular physiology, Vol. 232, Issue 1, pp. 167-75, (2016) (PubMed).

Karimian Azari, Leitner, Jaggi, Langhans, Mansouri: "Possible role of intestinal fatty acid oxidation in the eating-inhibitory effect of the PPAR-? agonist Wy-14643 in high-fat diet fed rats." in: PLoS ONE, Vol. 8, Issue 9, pp. e74869, (2013) (PubMed).

Target Details for HMGCS2

Target: HMGCS2 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial) (HMGCS2))

Alternative Name: HMGCS2

Background: HMGCS2 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Protein Interaction Partner: UBC, APP, YWHAQ,
Protein Size: 508

Molecular Weight: 56 kDa

Gene ID: 3158

NCBI Accession: NM_005518, NP_005509

UniProt: P54868

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha