Keratin 2 antibody (Middle Region)

Catalog No. ABIN2776963

The Rabbit Polyclonal anti-Keratin 2 antibody has been validated for WB and IHC. It is suitable to detect Keratin 2 in samples from Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Pig and Rabbit.

Quick Overview for Keratin 2 antibody (Middle Region) (ABIN2776963)

Target: Keratin 2 (KRT2)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Keratin 2 Antibody

Binding Specificity: Middle Region

Sequence: EVKAQYEEIA QRSKEEAEAL YHSKYEELQV TVGRHGDSLK EIKIEISELN

Predicted Reactivity: Cow: 79%, Dog: 100%, Guinea Pig: 79%, Horse: 88%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 79%, Rat: 81%

Characteristics: This is a rabbit polyclonal antibody against KRT2A. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KRT2A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 639 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Keratin 2

Target: Keratin 2 (KRT2)

Alternative Name: KRT2A

Background: KRT2A is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Alias Symbols: KRT2E, KRTE, K2e, CK-2e, KRT2A
Protein Interaction Partner: KRT40, KRT38, KRT31, KRT19, KRT13, Fbxl16, LGR4, EED, ADRB2, PAN2, Nphp4, IQCB1, VCAM1, UBC, FN1, EIF4A3, MAGOH, KRT10, KRT1, UBASH3B, SHC1, PIK3R2, INPPL1, GRB2, EPS15, CRK, AP2M1, CBL, CAND1, DCUN1D1, COPS5, COPS6, CUL1, CUL2, CUL3, CUL4A, CUL4B, CUL5,
Protein Size: 639

Molecular Weight: 70 kDa

Gene ID: 3849

NCBI Accession: NM_000423, NP_000414

UniProt: P35908