CYP3A7 antibody (Middle Region)

Catalog No. ABIN2776998

Product Details anti-CYP3A7 Antibody

Target: CYP3A7 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 7 (CYP3A7))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP3A7 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KSVKQIKEGR LKETQKHRVD FLQLMIDSQN SKDSETHKAL SDLELMAQSI

Predicted Reactivity: Cow: 77%, Dog: 75%, Guinea Pig: 83%, Human: 100%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against CYP3A7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CYP3A7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 503 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CYP3A7 antibody (ABIN2776998)

Van Peer, Verbueken, Saad, Casteleyn, Van Ginneken, Van Cruchten: "Ontogeny of CYP3A and P-glycoprotein in the liver and the small intestine of the Göttingen minipig: an immunohistochemical evaluation." in: Basic & clinical pharmacology & toxicology, Vol. 114, Issue 5, pp. 387-94, (2014) (PubMed).

Target Details for CYP3A7

Target: CYP3A7 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 7 (CYP3A7))

Alternative Name: CYP3A7 (CYP3A7 Products)

Synonyms: CP37 antibody, CYPIIIA7 antibody, P450-HFLA antibody, cytochrome P450, family 3, subfamily A, polypeptide 7 antibody, cytochrome P450 family 3 subfamily A member 7 antibody, CYP3A7 antibody

Background: CYP3A7 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. The enzyme also metabolizes some drugs such as aflatoxin B1.This gene, CYP3A7, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. The enzyme also metabolizes some drugs such as aflatoxin B1. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Transcript variants have been described, but it is not known whether these transcripts are normally produced.
Alias Symbols: CP37, P450-HFLA, CYPIIIA7
Protein Size: 503

Molecular Weight: 57 kDa

Gene ID: 1551

NCBI Accession: NM_000765, NP_000756

UniProt: P24462

Pathways: Steroid Hormone Biosynthesis