DLAT antibody (N-Term)

Catalog No. ABIN2777101

This Rabbit Polyclonal antibody specifically detects DLAT in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig and Horse.

Quick Overview for DLAT antibody (N-Term) (ABIN2777101)

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DLAT Antibody

Binding Specificity: N-Term

Sequence: WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against DLAT. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DLAT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 647 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DLAT

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Alternative Name: DLAT

Background: DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC, EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC, MIM 109720) show autoantibodies to DLAT.[supplied by OMIM].
Alias Symbols: DLTA, PDC-E2, PDCE2
Protein Interaction Partner: MDM2, UBC, ADRB2, PRKD3, PDHX, MMGT1, MRPL1, ACAD9, SYNCRIP, PDHA2, PDHA1, OGDH, NDUFS4, HSPA1L, DLD, COX5B, COPS5, CUL1, SIRT7, TSG101, KIAA0101, PSMA3, PDK4, PDK3, PDK2, PDK1, PDHB,
Protein Size: 647

Molecular Weight: 69 kDa

Gene ID: 1737

NCBI Accession: NM_001931, NP_001922

UniProt: P10515