Glucagon antibody (N-Term)

Catalog No. ABIN2777107

The Rabbit Polyclonal anti-Glucagon antibody has been validated for WB. It is suitable to detect Glucagon in samples from Human, Rat, Mouse, Cow, Dog, Sheep, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for Glucagon antibody (N-Term) (ABIN2777107)

Target: Glucagon (GCG)

Reactivity: Human, Rat, Mouse, Cow, Dog, Sheep, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucagon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glucagon Antibody

Binding Specificity: N-Term

Sequence: LSDPDQMNED KRHSQGTFTS DYSKYLDSRR AQDFVQWLMN TKRNRNNIAK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against GCG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GCG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 180 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: GCG

Background: GCG is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Alias Symbols: GLP1, GLP2, GRPP
Protein Interaction Partner: ARRB2, GLP1R, GLP2R, MEP1B, MEP1A, GCGR, CTSL, CPE,
Protein Size: 180

Molecular Weight: 20 kDa

Gene ID: 2641

NCBI Accession: NM_002054, NP_002045

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling