PPL antibody (Middle Region)

Catalog No. ABIN2777127

This Rabbit Polyclonal antibody specifically detects PPL in WB. It exhibits reactivity toward Human and Pig.

Quick Overview for PPL antibody (Middle Region) (ABIN2777127)

Target: PPL (Periplakin (PPL))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPL Antibody

Binding Specificity: Middle Region

Sequence: EKSRAQEKVT EKEVVKLQND PQLEAEYQQL QEDHQRQDQL REKQEEELSF

Predicted Reactivity: Human: 100%, Pig: 82%

Characteristics: This is a rabbit polyclonal antibody against PPL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1756 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPL

Target: PPL (Periplakin (PPL))

Alternative Name: PPL

Background: PPL is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: KIAA0568, MGC134872
Protein Interaction Partner: CEP57L1, ZSCAN1, RAB3IP, TSC22D4, RALBP1, AHCYL1, VIM, TRAF2, TRIM27, ALAS1, AKT1, SKIL, UBC, USP7, CUL1, ITSN1, Cdk1, KAZN, PPHLN1, EVPL, CASP6, OPRM1, COL17A1, KRT8, EZR,
Protein Size: 1756

Molecular Weight: 205 kDa

Gene ID: 5493

NCBI Accession: NM_002705, NP_002696

UniProt: O60437