TNNI1 antibody (N-Term)

Catalog No. ABIN2777151

The Rabbit Polyclonal anti-TNNI1 antibody has been validated for WB and IHC. It is suitable to detect TNNI1 in samples from Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse and Rabbit. There is 1 publication available.

Quick Overview for TNNI1 antibody (N-Term) (ABIN2777151)

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNNI1 Antibody

Binding Specificity: N-Term

Sequence: MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 79%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TNNI1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TNNI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 187 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TNNI1 antibody (ABIN2777151)

Salzler, Shah, Doré, Bauerlein, Miloscio, Latres, Papadopoulos, Olson, MacDonald, Duan: "Myostatin deficiency but not anti-myostatin blockade induces marked proteomic changes in mouse skeletal muscle." in: Proteomics, Vol. 16, Issue 14, pp. 2019-27, (2016) (PubMed).

Target Details for TNNI1

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Alternative Name: TNNI1

Background: Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 Molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
Alias Symbols: DKFZp451O223, TNN1, SSTNI
Protein Interaction Partner: PNMA1, MDFI, TRIM63, TRIM55, TNNC1, TNNT2, EIF3I, CCDC85B, TNNT1, ACTA1, PKD2L1,
Protein Size: 187

Molecular Weight: 21 kDa

Gene ID: 7135

NCBI Accession: NM_003281, NP_003272

UniProt: P19237