Nanog antibody (Middle Region)

Catalog No. ABIN2777175

This Rabbit Polyclonal antibody specifically detects Nanog in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Sheep, Goat, Horse, Rabbit and Guinea Pig and has been mentioned in 4+ publications.

Quick Overview for Nanog antibody (Middle Region) (ABIN2777175)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Goat, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: Middle Region

Sequence: SYKQVKTWFQ NQRMKSKRWQ KNNWPKNSNG VTQKASAPTY PSLYSSYHQG

Predicted Reactivity: Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 86%, Rat: 92%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NANOG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 305 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nanog antibody (ABIN2777175)

Kallas, Pook, Maimets, Zimmermann, Maimets: "Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells." in: PLoS ONE, Vol. 6, Issue 4, pp. e19114, (2011) (PubMed).

Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, (2010) (PubMed).

Neganova, Zhang, Atkinson, Lako: "Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells." in: Oncogene, Vol. 28, Issue 1, pp. 20-30, (2009) (PubMed).

Maimets, Neganova, Armstrong, Lako: "Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells." in: Oncogene, Vol. 27, Issue 40, pp. 5277-87, (2008) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305

Molecular Weight: 34 kDa

Gene ID: 79923

NCBI Accession: NM_024865, NP_079141

UniProt: Q6NSW7

Pathways: Stem Cell Maintenance