WT1 antibody (N-Term)

Catalog No. ABIN2777189

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in WB. Suitable for Human, Rat, Mouse, Rabbit, Dog, Pig and Zebrafish (Danio rerio).

Quick Overview for WT1 antibody (N-Term) (ABIN2777189)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse, Rabbit, Dog, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: N-Term

Sequence: DFAPPGASAY GSLGGPAPPP APPPPPPPPP HSFIKQEPSW GGAEPHEEQC

Predicted Reactivity: Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against WT1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human WT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.
Protein Interaction Partner: KRTAP10-3, KRTAP10-8, KRT40, EGR1, MDM2, DVL3, CIAO1, TP53, HSPA4, TAOK1, NPM3, ZNF205, SUZ12, MEN1, EZH2, DNMT1, WTIP, WTAP, PAWR, UBE2I, TP63, FHL2, TP73, PRKACA, CREBBP, U2AF2, PAX2, AREG,
Protein Size: 432

Molecular Weight: 47 kDa

Gene ID: 7490

Pathways: Tube Formation