E2F4 antibody (C-Term)

Catalog No. ABIN2777218

The Rabbit Polyclonal anti-E2F4 antibody has been validated for WB and IHC. It is suitable to detect E2F4 in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for E2F4 antibody (C-Term) (ABIN2777218)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-E2F4 Antibody

Binding Specificity: C-Term

Sequence: SSELLEELMS SEVFAPLLRL SPPPGDHDYI YNLDESEGVC DLFDVPVLNL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against E2F4. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 413 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Alias Symbols: E2F-4
Protein Interaction Partner: RBL2, TFDP1, RBL1, RB1, XPO1, PCM1, HDAC1, POLR1D, CHMP2A, AP3S2, TRIM28, LIN9, ARID3A, NCOR2, HDAC3, AR, CEBPA, LIN54, LIN37, UBC, ATAD2, SIN3A, ASH2L, HCFC1, TRRAP, KAT2A, ID2, E2F1, KMT2A, ID3, SMAD3, NOLC1, TFDP2, NDN, SMAD2, BRCA1, RYBP, RNF144A, FHL
Protein Size: 413

Molecular Weight: 44 kDa

Gene ID: 1874

NCBI Accession: NM_001950, NP_001941

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size