PAX2A antibody (Middle Region)

Catalog No. ABIN2777232

The Rabbit Polyclonal anti-PAX2A antibody has been validated for WB. It is suitable to detect PAX2A in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit, Sheep, Guinea Pig and Horse. There are 6+ publications available.

Quick Overview for PAX2A antibody (Middle Region) (ABIN2777232)

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit, Sheep, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAX2A Antibody

Binding Specificity: Middle Region

Sequence: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PAX2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human PAX2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 393 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PAX2A antibody (ABIN2777232)

Palmieri, Gojis, Rudraraju, Stamp-Vincent, Wilson, Langdon, Gourley, Faratian: "Expression of steroid receptor coactivator 3 in ovarian epithelial cancer is a poor prognostic factor and a marker for platinum resistance." in: British journal of cancer, Vol. 108, Issue 10, pp. 2039-44, (2013) (PubMed).

Viringipurampeer, Ferreira, DeMaria, Yoon, Shan, Moosajee, Gregory-Evans, Ngai, Gregory-Evans: "Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during eye development." in: Human molecular genetics, Vol. 21, Issue 10, pp. 2357-69, (2012) (PubMed).

Lee, Doberstein, Baumgarten, Wieland, Ungerer, Bürger, Hardt, Boehncke, Pfeilschifter, Mihic-Probst, Mittelbronn, Gutwein: "PAX2 regulates ADAM10 expression and mediates anchorage-independent cell growth of melanoma cells." in: PLoS ONE, Vol. 6, Issue 8, pp. e22312, (2011) (PubMed).

Yu, Moriniere, Birke, Neumann, Fuchshofer, Kampik, Bloemendal, Welge-Lussen: "Reactivation of optic nerve head astrocytes by TGF-beta2 and H2O2 is accompanied by increased Hsp32 and Hsp47 expression." in: Investigative ophthalmology & visual science, Vol. 50, Issue 4, pp. 1707-17, (2009) (PubMed).

Hurtado, Holmes, Geistlinger, Hutcheson, Nicholson, Brown, Jiang, Howat, Ali, Carroll: "Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen." in: Nature, Vol. 456, Issue 7222, pp. 663-6, (2008) (PubMed).

Yu, Fuchshofer, Birke, Priglinger, Eibl, Kampik, Bloemendal, Welge-Lussen: "Hypoxia/reoxygenation and TGF-beta increase alphaB-crystallin expression in human optic nerve head astrocytes." in: Experimental eye research, Vol. 84, Issue 4, pp. 694-706, (2007) (PubMed).

Target Details for PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Alternative Name: PAX2

Background: PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia.
Alias Symbols: -
Protein Interaction Partner: BBS7, BBS4, BBS2, BBS1, RB1, BRCA1, SFRP2, PAXIP1, WT1, MAPK8IP1, MAPK8, ID2,
Protein Size: 393

Molecular Weight: 42 kDa

Gene ID: 5076

NCBI Accession: NM_000278, NP_000269

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation