NFYA antibody (C-Term)

Catalog No. ABIN2777243

The Rabbit Polyclonal anti-NFYA antibody has been validated for WB and IHC. It is suitable to detect NFYA in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae.

Quick Overview for NFYA antibody (C-Term) (ABIN2777243)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFYA Antibody

Binding Specificity: C-Term

Sequence: YLHESRHRHA MARKRGEGGR FFSPKEKDSP HMQDPNQADE EAMTQIIRVS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 80%

Characteristics: This is a rabbit polyclonal antibody against NFYA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NFYA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 347 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA

Background: NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
Alias Symbols: HAP2, CBF-A, CBF-B, NF-YA
Protein Interaction Partner: LUC7L2, POGZ, NFYB, GTF2A1, CSNK2A1, UBC, APPBP2, TP63, TP53, NFYC, EP300, CDK2, CCNA2, CDC25A, APP, RPS6KA6, JUN, HDAC1, ATF2, SP1, TAF6, TAF11, TAF12, PAPOLG, PWP1, SREBF2, ZHX2, ZHX1, ZHX3, KPNB1, SRSF1, HMGA1, SRF, USF2, USF1, NFYA, NR5A1, POU2F1, CEB
Protein Size: 347

Molecular Weight: 37 kDa

Gene ID: 4800

NCBI Accession: NM_002505, NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha