SUPT16H antibody (N-Term)

Catalog No. ABIN2777339

The Rabbit Polyclonal anti-SUPT16H antibody has been validated for WB. It is suitable to detect SUPT16H in samples from Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for SUPT16H antibody (N-Term) (ABIN2777339)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SUPT16H Antibody

Binding Specificity: N-Term

Sequence: ASKKKVEFLK QIANTKGNEN ANGAPAITLL IREKNESNKS SFDKMIEAIK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SUPT16H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SUPT16H

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 602 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H.
Alias Symbols: CDC68, FACT, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68
Protein Interaction Partner: HIST1H2AB, UBC, SUMO2, ATRX, SUMO1, NEDD8, RNF20, PAF1, EED, RNF2, BMI1, rev, FTH1, QRICH1, SRPK2, ICAM1, CD81, IGSF8, PNKP, VCP, FMNL1, SSRP1, ESR1, CENPA, LEO1, RBM25, PRPF6, NCSTN, RTF1, ACIN1, PSIP1, USP39, PRPF8, SF3A1, SAP18, CTR9, DDX21, PRPF3, PRP
Protein Size: 602

Molecular Weight: 66 kDa

Gene ID: 11198

NCBI Accession: NM_007192

UniProt: Q9Y5B9

Pathways: Chromatin Binding