HIC2 antibody (Middle Region)

Catalog No. ABIN2777438

The Rabbit Polyclonal anti-HIC2 antibody has been validated for WB and IF. It is suitable to detect HIC2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit. There is 1 publication available.

Quick Overview for HIC2 antibody (Middle Region) (ABIN2777438)

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HIC2 Antibody

Binding Specificity: Middle Region

Sequence: CKEEEENGKD ASEDSAQSGS EGGSGHASAH YMYRQEGYET VSYGDNLYVC

Predicted Reactivity: Cow: 85%, Dog: 85%, Guinea Pig: 85%, Horse: 85%, Human: 100%, Mouse: 85%, Rabbit: 77%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against HIC2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HIC2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 615 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HIC2 antibody (ABIN2777438)

Collins, Wright, Edwards, Davis, Grinham, Cole, Goward, Aguado, Mallya, Mokrab, Huckle, Beare, Dunham: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, (2004) (PubMed).

Target Details for HIC2

Target: HIC2 (Hypermethylated in Cancer 2 (HIC2))

Alternative Name: HIC2

Background: HIC2 contains 5 C2H2-type zinc fingers and 1 BTB (POZ) domain. It belongs to the krueppel C2H2-type zinc-finger protein family, Hic subfamily and is a transcriptional repressor.
Alias Symbols: HRG22, KIAA1020, ZBTB30, ZNF907
Protein Interaction Partner: HIC2, HIC1, BARD1, LIG4, APP, PDK2, CCNT1,
Protein Size: 615

Molecular Weight: 66 kDa

Gene ID: 23119

NCBI Accession: NM_015094, NP_055909

UniProt: Q96JB3