MBD1 antibody (C-Term)

Catalog No. ABIN2777532

This Rabbit Polyclonal antibody specifically detects MBD1 in WB and IHC. It exhibits reactivity toward Human, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for MBD1 antibody (C-Term) (ABIN2777532)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reactivity: Human, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MBD1 Antibody

Binding Specificity: C-Term

Sequence: EEDKEENKDD SASKLAPEEE AGGAGTPVIT EIFSLGGTRF RDTAVWLPRS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MBD1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 629 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1

Background: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions, several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides, transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Alias Symbols: CXXC3, PCM1, RFT
Protein Interaction Partner: SUMO1, HTT, UBC, ECT2, APP, ATXN1L, TENM1, SUMO2, HIST1H3A, PCNA, ATF7IP2, ATF7IP, CHAF1A, SETDB1, PIAS1, STAT1, PRAM1, HDAC3, PIAS2, ZNF512B, Rnf2, CBX4, CBX5, HIPK3, SMAD9, OASL, SMAD5, SMAD3, SMAD7, SMAD1, SUV39H1,
Protein Size: 629

Molecular Weight: 69 kDa

Gene ID: 4152

NCBI Accession: NM_001204138, NP_001191067

UniProt: Q9UIS9