NFIX antibody (Middle Region)

Catalog No. ABIN2777545

The Rabbit Polyclonal anti-NFIX antibody has been validated for WB and IHC. It is suitable to detect NFIX in samples from Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for NFIX antibody (Middle Region) (ABIN2777545)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFIX Antibody

Binding Specificity: Middle Region

Sequence: YFVHTPESGQ SDSSNQQGDA DIKPLPNGHL SFQDCFVTSG VWNVTELVRV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NFIX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFIX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NFIX

Background: Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.
Alias Symbols: NF1A
Protein Interaction Partner: QRICH1, NFIX, ZNF614, SUMO1, UBC, NEDD8, JMJD6, ISG15, NFIB, PIR, RFX1, SKI, RB1, HDAC1,
Protein Size: 441

Molecular Weight: 49 kDa

Gene ID: 4784

NCBI Accession: NM_002501, NP_002492

Pathways: Skeletal Muscle Fiber Development